This is the thoroughly revised edition of a comprehensive text–resource that delves into the fundamental principles and complex processes of biochemistry. It completely covers all the topics related to the competencies as per the latest MC guided Competency Based Medical Education Curriculum under Graduate Medical Education Regulation. Structure and function of macromolecules such as proteins, nucleic acids, carbohydrates and lipids are adequately covered. The book also explores enzymatic reactions, metabolic pathways and the molecular basis of genetic information transfer. Concepts related to the immunological basis of health and disease have been covered in detail. The chapter on nutrition has been completely overhauled as per ICMR-NIN (2020) guidelines to give it an updated face. The detailed approach of the book provides a solid foundation for students and researchers, offering an insight into both the classical biochemical concepts and the latest advancements in the field. One of the key features of this textbook is its emphasis on the practical applications of biochemistry in fields like medicine, pharmacology and biotechnology. It includes numerous illustrations, diagrams and the real-world examples which enhance the understanding and retention of complex biochemical phenomena. Additionally, the textbook also integrates clinical correlations to illustrate the relevance of biochemical knowledge in diagnosing and treating diseases. This blend of theory and application makes